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. 2018 Oct 8;8:14958. doi: 10.1038/s41598-018-33352-8

Figure 6.

Figure 6

Beauvericin upregulates the expression of liver X receptor-α (LXR-α). (A) B16F10 cells were co-transfected with the liver X receptor (LXR)-α promoter-luciferase reporter and β-galactosidase reporter vector using PEI transfection reagent. After 24 h, transfected cells were incubated with the indicated concentrations of beauvericin. LXR-α promoter-luciferase reporter activity was determined. β-galactosidase assay was used to measure transfection efficiency. Results were confirmed from at least three independent experiments and values represent the means ± SEM. P-values were obtained by the one-way ANOVA. *P < 0.05 vs. untreated control. (B) Cells were treated with the indicated concentrations of beauvericin for 24 h. Cells were harvested, lysed, and the protein level of LXR-α was examined by Western blot analysis. Results were confirmed from at least three independent experiments, and the values represent the means ± SEM. P-values were obtained by the one-way ANOVA. *P < 0.05 versus untreated control. BEA, beauvericin.