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. 2018 Oct 8;8:14966. doi: 10.1038/s41598-018-33378-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Establishment of the screening system. (A) Principle of the RUSH-secretion assay. In the absence of biotin, ss-SBP-GFP reporter is retained in the ER by the streptavidin-KDEL hook. Upon biotin addition, ss-SBP-GFP reporter leaves the ER and accumulates in the Golgi before being released from the cell. (B) Time lapse microscopy in the absence or presence of biotin depicts the phenotypic change of cellular florescence. (C) Counterstaining and immunostaining of streptavidin in fixed cells preincubated or not with biotin for 4 h. Scale bar equals 10 μm.