Fig. 4.

Influence of non-coding intron 4 ctc versus ∆ctc on A3H minigene expression. a Schematic of the A3H minigene construct with key elements labeled. The minigene haplotype I intron has ctc (green) and the haplotype II intron has ∆ctc (blue). These constructs are otherwise identical. b Immunoblots of HeLa and MCF7 whole-cell extracts expressing the indicated A3H minigene constructs (representative of two independent experiments). Low and high exposures are provided to show that SV200 expression is specific to the haplotype II minigene. An anti-TUBULIN (TUB) blot is shown as a loading control