Figure 10.

Stability of DPP in the solution at pH 7 under the presence or absence of collagen. (A) SDS-PAGE stained with CBB or CBB plus Stains-all. DPP was not stained with CBB but can be visualized with Stains-all. DPP is not stable when incubated at higher temperature. (B) SDS-PAGE of Col I and DPP after being mixed together and incubated at 4, 20, 37, or 95 °C. The presence of collagen reduces the instability of DPP at 37 and 95 °C. (C) SDS-PAGE showing type I collagen (Col I). Col I stains with CBB but does not stain with Stains-all (SA).