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. 2018 Oct 2;8:330. doi: 10.3389/fcimb.2018.00330

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Copyright © 2018 Abhishek, Saikia, Gupta, Bansal, Gupta, Singh, Laal and Verma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LAMP-1 staining for lysosomal localization of M. tuberculosis in RPE (ARPE-19) cells. Confocal micrographs of uninfected (UI) (A) and infected (I) ARPE-19 cells (MOI 10:1) at 0-dpi (B), 1-dpi (C), and 3-dpi (D) showing staining for (i) M. tuberculosis labeled with PKH26 dye (red channel); (ii) LAMP-1 antibody using secondary FITC-IgG (green channel); (iii) DAPI for nuclei (blue channel). In infected ARPE-19 cells (rows B–D; column iv), white arrowheads show merging of two channels (red and green) and the Pearson's R coefficient for the colocalization of M. tuberculosis with LAMP-1. Zero is no colocalization, and 1 means perfect colocalization. Dpi, day post-infection; UI, uninfected; I, infected.