Table 3.

Mycobacterium tuberculosis transcripts in vitreous samples from intraocular tuberculosis patients.

Samples categories	Sample ID	Rv1230c	Rv1971
Possible IOTB	H-IOTB-S4	D+	–
	H-IOTB-S5	R+	–
	H-IOTB-S9	D+	D+
Confirmed IOTB	H-IOTB-S11	–	–
	H-IOTB-S12	D+	D+
	H-IOTB-S13	D+	D+
	H-IOTB-S14	R+	D+
	H-IOTB-S15	D+	D+
	H-IOTB-S16	R+	R+
	H-IOTB-S17	R+	D+

Human vitreous samples from two different categories of IOTB were examined for M. tuberculosis transcripts corresponding to Rv1230c, Rv1971, and Rv3872 genes using H37Rv (as a positive control). Transcripts which showed C_T value in RT+ sample were denoted as R+ (as the C_T was due to cDNA synthesized from RNA), while early C_T value in RT- of the same sample was denoted as D+ (as the C_T was due to the presence of DNA). Rv3872 is negative for all clinical samples at both RNA and DNA levels. IOTB, intraocular tuberculosis; cDNA, complementary deoxyribonucleic acid; RT, reverse transcriptase; RT-PCR, real-time polymerase chain reaction; H-IOTB, all clinical samples identity number. +, Positive; –, Negative.