FIGURE 12.

Bacterial two-hybrid analysis of FtsZ and ΔN-FtsZ interactions. Interactions of T25- and T18-fusion proteins produced in E. coli were measured as β-galactosidase activity in liquid cultures. The topology of the fusion is indicated by the order of components (T18-protein and T25-protein denotes the corresponding adenylate cyclase domain fused to the N-terminus of the tested protein; protein-T18 and protein-T25 denotes the corresponding adenylate cyclase domain fused to the C-terminus of the tested protein). Values (nmol ONPG⋅min^-1⋅mg prot^-1) are given as mean ± standard deviation. The significance of differences as assessed by Student’s t-tests is shown (^∗∗p < 0.01; values with regard to the two respective controls: bacteria containing one of the two plasmids encoding a fused gene and the complementary empty vector) (see Supplementary Table S2 for further details).