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. 2018 Oct 2;9:2260. doi: 10.3389/fmicb.2018.02260

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Copyright © 2018 Corrales-Guerrero, Camargo, Valladares, Picossi, Luque, Ochoa de Alda and Herrero.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Polymerization of Anabaena FtsZ and ΔN-FtsZ proteins. (A) 3 μM of FtsZ or ΔN-FtsZ protein was incubated in the presence of 2 mM GTP under the conditions described in Section “Materials and Methods”. At the indicated times aliquots of the incubation mixtures were centrifuged at 150,234 g during 15 min. Aliquots of the resulting supernatant and precipitated fractions were resolved in SDS-PAGE gels, and bands were scanned and quantified. The numbers at the bottom of gels represent the % of total protein found in the sediment. Approximate size (kDa) correspondence of molecular weight markers is indicated at left. (B) The average values of two independent experiments with very similar results are represented.