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. 2018 Oct 2;9:2125. doi: 10.3389/fimmu.2018.02125

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Copyright © 2018 Garcia-Carmona, Ting, Radigan, Athuluri Divakar, Chavez, Meffre, Cerutti and Cunningham-Rundles.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) TACI variants with diverse deleted extracellular domains retain capacity for complex assembly. Upper panel shows HA labeled-TACI deletion mutant products: Full length TACI-L, TACI-S (minus CRD1), exon 1 and CRD1 (ΔE1-CRD1), CRD2 (ΔCDR2), minus the remaining extracellular 59 amino acids (Δaa105-164) and excision of both CRD1 and CRD2 (Δ21-104) were constructed. (B) Constructs were transfected into HEK-293T cells along with TACI-L-FLAG; after cell lysis, precipitates were harvested with anti-FLAG sepharose beads, and complexes in lysates examined after PAGE and immunoblotting using an anti-HA antibody. FLAG expression was tested as control (lower panel). Data are representative of 3 independent experiments.