Figure 2.

Serum reduced NET formation of isolated PMN. In serum-containing medium (R10) all SPIONs (200 μg/ml) are colloidally stable (A). Isolated PMN were incubated with 200 μg/ml nanoparticles for 3 h in R10. Then, cells were stained with Hoechst, and prepared for fluorescence microscopy. PMA treated and untreated cells served as positive and negative control, respectively. Scale bars refer to 50 μm (B). Measurement of neutrophils by flow cytometry and evaluation of viable PMN count based on forward and side scatter properties. Experiment was performed in triplicates of at least two independent donors; representative data of one donor (mean values with standard deviations) are shown (C).