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. 2018 Oct 2;11:353. doi: 10.3389/fnmol.2018.00353

Figure 3.

Figure 3

Activation of 5-HT7 receptors reversed mGluR-LTD by stimulation of adenylate cyclase. (A) In hippocampal WT slices, application of LP-211 did not modify mGluR-LTD in the presence of the adenylate cyclase blocker SQ 22536 (10 μM, included in intracellular pipette solution; dark gray dots, n = 6). (B) The bar graph shows the amount of mGluR-LTD measured 45 min after LTD induction (mean EPSC amplitude in all tested neurons, expressed as % of baseline EPSC amplitude). In WT slices, bath applications of DHPG-induced mGluR-LTD (white column; n = 11) that was completely reversed when DHPG application was followed by application of the 5-HT7R agonist LP-211 (10 nM, 5 min; black column, n = 7; *P = 0.03 by unpaired t-test). In the presence of intracellular SQ 22536 (10 μM; gray column, n = 6), the amount of mGluR-LTD was slightly increased with respect to control and was not reversed by application of LP-211 (10 nM, 5 min; dark gray column, n = 6; significantly different from the effect of LP-211 in control conditions, **P = 0.0026 by unpaired t-test). (C) Similarly, in Fmr1 KO slices application of LP-211 (10 nM, 5 min) had no effect on mGluR-LTD in the presence of intracellular SQ 22536 (10 μM; dark gray column, n = 6). (D) In Fmr1 KO slices, application of LP-211 reversed DHPG-induced mGluR-LTD only in control conditions (black column, n = 6; *P = 0.02 by unpaired t-test) but not in the presence of SQ 22536 (SQ, 10 μM; gray column, n = 6), showing that 5-HT7R-mediated effect required stimulation of adenylate cyclase also in Fmr1 KO slices.