Figure 4.

The NK1.1⁺ population among CD8⁺ T cells is mainly responsible for colitis in Yeti mice. (A) The percentage of NK1.1⁺CD8⁺ T cells among total CD8⁺ T cells and NK1.1⁺ cell-depleted CD8⁺ T cells from the MLN of Yeti/CD1d KO mice was determined. (B–D) Either total CD8⁺ T cells (2 × 10⁶) or NK1.1⁻CD8⁺ T cells (2 × 10⁶) from Yeti/CD1d KO mice were i.v. transferred to CD1d KO mice. Daily body weight changes, DAI score (B), and colon length (C) of each group were evaluated after 1.5% DSS treatment. (D) Intracellular IFNγ and IL17 production and the frequencies of Foxp3⁺CD25⁺ cells were assessed in the MLN and LP CD4⁺ T cells from these mice by flow cytometry on day 10. The mean values ± SD (n = 5 per group in the experiment; Student's t-test; ^*P < 0.05, ^**P < 0.01) are shown.