Table I.
CYP2D6 variant primers, product lengths and enzymes used for polymerase chain reaction-restriction fragment length polymorphism analysis.
| Variant | rs no. | Primers, 5–3 | Product length, bp | Enzyme | Refs. |
|---|---|---|---|---|---|
| CYP2D6*3 | rs35742686 | F-ATGAGCTGCTAACTGAGCCC | 270 | MspI | (43) |
| R-CCGAGAGCATACTCGGGAC | |||||
| CYP2D6*4 | rs3892097 | F-TGCCGCCTTCGCCAACCACT | 309 | BstNI | (26) |
| R-TCGCCCTGCAGAGACTCCTC | |||||
| CYP2D6*10 | Rs1065852 | F-GTGCTGAGAGTGTCCTGCC | 344 | HphI | (26) |
| R-CACCCACCATCCATGTTTGC | |||||
| CYP2D6*17 | rs28371706 | F-CGGTGGTCGTGCCTCAATG | 167 | HphI | (44) |
| R-CCCGGGTCCCACGGAAATCT |
CYP2D6, cytochrome P450 2D6; rs, reference single nucleotide polymorphism; F, forward; R, reverse.