Figure 2.

Effects of Malat1 on the maturation of AEC-treated DCs. AECs were transfected with NC siRNA or Malat1 siRNA and then cocultured with DCs for 72 h. The costimulatory molecules (A and B) CD80, (C and D) CD86, and (E and F) MHCII in DCs were detected by FACS. (G) Relative mRNA levels of CD80, CD86, and MHCII in DCs were determined by real-time PCR (n=5, *P<0.05 vs. ‘NC siRNA’ group). AEC, airway epithelial cells; DC, dendritic cell; Malat1, metastasis-associated lung adenocarcinoma transcript 1; PCR, polymerase chain reaction.