Figure 3.

Effects of Malat1 on the inflammatory response of AEC-treated DCs. AECs were incubated in the presence of NC siRNA or Malat1 siRNA and then cocultured with DCs for further 72 h. The expression of TNF-α, IL-4, IL-6, and IFN-r in DCs were regulated after the suppression of Malat1. Relative mRNA levels of (A) TNF-α, (B) IL-4, (C) IL-6, and (D) IFN-r detected by RT-qPCR. The protein levels of (E) TNF-α, (F) IL-4, (G) IL-6, and (H) IFN-r were detected by ELISA. Mean ± SD was obtained from five different experiments (*P<0.05 vs. ‘NC siRNA’ group). AEC, airway epithelial cells; DC, dendritic cell; Malat1, metastasis-associated lung adenocarcinoma transcript 1; RT-qPCR, real-time quantitive polymerase chain reaction; TNF-α, tumor necrosis factor-α.