Figure 4.

Effects of Malat1 on the chemokine expression of AEC-treated DCs. AECs were incubated in the presence of the NC siRNA or Malat1 siRNA and then cocultured with DCs for 72 h. Relative mRNA level of (A) CXCR2 and (B) CXCR4 as detected by RT-qPCR. Relative protein level of (C) CXCR2 and (D) CXCR4 as detected by ELISA. (E) CI of the Transwell results. Mean ± SD calculated from five different experiments (*P<0.05 vs. ‘NC siRNA’ group). CI, chemotaxis index; AEC, airway epithelial cells; DC, dendritic cell; Malat1, metastasis-associated lung adenocarcinoma transcript 1; RT-qPCR, real-time quantitive polymerase chain reaction.