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. 2018 Sep 4;16(5):4042–4048. doi: 10.3892/etm.2018.6691

Figure 4.

Figure 4.

Hydrogen inhibited ox-LDL-induced inflammatory cytokine expression by upregulation of autophagic flux in RAW264.7 cells. The expression of (A) TNF-α, (B) IL-6 and (C) IL-1β after RAW264.7 cells were incubated with/without hydrogen (0.00, 0.15, 0.30 and 0.60 mM), prior to being stimulated with/without ox-LDL (100 mg/l) for 24 h. The expression of (D) TNF-α, (E) IL-6 and (F) IL-1β after RAW264.7 cells were incubated with/without hydrogen (0.60 mM), bafilomycin A1 (50 nM) and/or rapamycin (200 nM), prior to being stimulated with/without ox-LDL (100 mg/l) for 24 h. The cytokine concentrations were quantified by ELISA. Data are presented as the mean ± standard error of the mean from three independent experiments. *P<0.05 as indicated. IL, interleukin; BafA1, bafilomycin A1; Rap, rapamycin; TNF-α, tumor necrosis factor-α; Ox-LDL, oxidized low-density lipoprotein.