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. 2018 Sep 1;8(9):1801–1811.

Figure 2.

Figure 2

ATG7 is required for autophagy in response to NBAT1. (A and B) The mRNA (A) and protein (B) levels of Beclin1, ATG5 and ATG7 were detected in control and NBAT1-overexpressing 95D cells. (C and D) The mRNA (C) and protein (D) levels of Beclin1, ATG5 and ATG7 were detected in control and NBAT1 knockdown A549 cells. (E) LC3 and GAPDH were analyzed by western blot. Representative western blot and densitometric analysis normalized to GAPDH demonstrating the effect of ATG7 siRNA on LC3-II levels increased by NBAT1 knockdown. (F) The effects of ATG7 siRNA on LC3 dots accumulation induced by NBAT1 silencing. *P < 0.05.