Figure 9.

c-Fos accumulation in HEK 293a cells expressing recombinant B₂Rs (A) or B₁Rs (B) and stimulated for 60 min with synthetic peptides or extracts from healthy subject blood samples incubated in the presence of tPA with or without enalaprilat (blood processed as in Figure 3). Presentation as in Figure 7. In (A), densitometry values significantly differed between them (ANOVA P < 10⁻⁴, ^*P < 0.05; ^**P < 0.001 for comparison with the common control value, Dunnett's test). In (B), densitometry values significantly differed between them (ANOVA P < 10⁻⁴, ^*P < 0.001 for comparison with the common control value, Dunnett's test). (C) The same assay based on recombinant B₂Rs was applied to extracts from HAE blood samples incubated in the presence of tPA with or without enalaprilat. Densitometry values significantly differed between them (ANOVA P < 10⁻⁴, ^*P < 0.05; ^**P < 0.001 for comparison with the common control value, Dunnett's test).