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. 2018 Oct 2;9:2289. doi: 10.3389/fimmu.2018.02289

Figure 1.

Figure 1

Tgr5 is an interferon-stimulated gene (ISG). (A,B) Quantitative PCR (Q-PCR) analysis of Tgr5 expression in PEMs infected with RNA virus VSV [1 MOI (multiplicity of infection), A] and DNA virus HSV-1 (1 MOI, B) for the indicated times. (C) Q-PCR analysis of Tgr5 expression in PEMs infected with the indicated VSV MOI for 8 h. (D) Q-PCR analysis of Tgr5 expression in RAW264.7 cells and BMMs infected with VSV (1 MOI) for 8 h. (E,F) Q-PCR analysis of Tgr5 expression in PEMs (E) and BMMs (F) stimulated with IFN-β (100 ng/ml) for the indicated hours. (G) Q-PCR analysis of Stat1 expression in PEMs transfected with Stat1 siRNA for 48 h. NC, negative control. (H,I) Q-PCR analysis of Tgr5 expression in PEMs transfected with Stat1 siRNA for 48 h and then stimulated with IFN-β (100 ng/ml) for 2 h (H) or infected with VSV (1 MOI) for 8 h (I). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal control for Q-PCR. The data are shown as the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001. All experiments were performed three times with similar results.