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. 2018 Oct 2;9:2289. doi: 10.3389/fimmu.2018.02289

Figure 2.

Figure 2

Overexpression of TGR5 inhibits virus replication. (A) Q-PCR analysis of TGR5 expression in HEK-293T cells transfected for 28 h with different amounts (0.6 and 1.8 μg) of TGR5 expression plasmid. Emv, empty vector. (B) HEK-293T cells transfected with TGR5 plasmid as described in (A) were infected with VSV (0.1 MOI) for 8 h and then observed under a microscope. Original magnification 10 × . (C) Q-PCR analysis of VSV RNA replicates in (B). (D,E) Q-PCR analysis of VSV RNA replicates in TGR5-overexpressing (1.8 μg) HEK-293T cells infected by VSV with the indicated MOI (D) and times (E). (F) Q-PCR analysis of Newcastle disease virus (NDV) RNA replicates in HEK-293T cells transfected with TGR5 plasmids as described in (A) and infected with NDV (0.25 MOI) for 12 h. (G) Q-PCR analysis of NDV RNA replicates in TGR5-overexpressing (1.8 μg) HEK-293T cells infected by NDV with the indicated MOI for 18 h. (H) Q-PCR analysis of herpes simplex virus (HSV-1) UL-30 expression in HEK-293T cells transfected with TGR5 plasmids as described in (A) and infected with HSV-1 (0.5 MOI) for 18 h. (I) Q-PCR analysis of HSV-1 UL-30 expression in TGR5-overexpressing (1.8 μg) HEK-293T cells infected by HSV-1 with the indicated MOI for 18 h. GAPDH was used as an internal control for Q-PCR. The data are shown as the mean ± SD. ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001. All experiments were performed three times with similar results.