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. 2018 Oct 2;9:2289. doi: 10.3389/fimmu.2018.02289

Figure 5.

Figure 5

TGR5 positively regulates IFN-I production. (A,B) Q-PCR analysis of Ifn-β (A) and Ifn-a4 (B) expression in Tgr5+/+- and Tgr5−/−-PEMs infected with VSV (1 MOI) for the indicated times. (C) Q-PCR analysis of Ifn-β expression in Tgr5+/+- and Tgr5−/−-PEMs infected with the indicated VSV MOI for 8 h. (D) Q-PCR analysis of Ifn-β expression in Tgr5+/+- and Tgr5−/−-BMMs infected with VSV (1 MOI) for the indicated times. (E) Q-PCR analysis of IFN-β expression in TGR5-overexpressing (1.8 μg) HEK-293T cells infected with VSV (1 MOI) for 8 h. (F,G) Q-PCR analysis of Ifn-β expression in Tgr5+/+- and Tgr5−/−-PEMs transfected with Poly (I:C) (1.0 μg/ml) (F) or stimulated with Poly (I:C) (10 μg/ml) (G) for the indicated times. (H) Q-PCR analysis of Ifn-β expression in Tgr5+/+- and Tgr5−/−-BMMs stimulated with Poly (I:C) (10 μg/ml) for the indicated times. (I) Q-PCR analysis of Ifn-β expression in PEMs pretreated with INT-777 (500 μM) for 1 h and then stimulated with Poly (I:C) (10 μg/ml) for 2 h. (J) Q-PCR analysis of Ifn-β expression in PEMs pretreated with INT-777 (500 μM) for 1 h and then infected with VSV (1 MOI) or HSV-1 (1 MOI) for 8 h. (K) ELISA of IFN-β in sera from Tgr5+/+- and Tgr5−/−-mice intraperitoneally injected with VSV (1 × 108 pfu per mouse) for 24 h. nd, not detected. (L) Q-PCR analysis of Ifn-β expression in organs from Tgr5+/+- and Tgr5−/−-mice in (K). GAPDH was used as an internal control for Q-PCR. The data are shown as the mean ± SD. ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001. All experiments were performed three times with similar results.