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. 2018 Apr 27;5(3):226–234. doi: 10.1016/j.gendis.2018.04.004

Figure 1.

Figure 1

LPS activated inflammation and autophagy in dose- and time-dependence in RAW264.7 cells. (A) RAW264.7 macrophages were treated with LPS with different concentrations (0, 10, 100, 500 ng/mL) for 24 h. And realtime PCR was used to detect the mRNA expression of various inflammatory factors such as TNFα, IL6, NOS2, COX2. GAPDH was performed as a controls. (B) Quantitative analysis of endogenous IL6,TNFα, NOS2 and COX2 expressions were shown in bar graphs. Data are showed as mean ± SEM and repeated for three times. (C) Cells were exposed to LPS with 500 ng/ml at different interval times (0,6,12,18,24 h), LC3 and BRF1 protein expression was examined by Western bloting. β-actin expression was regarded as control for similar loading of proteins in each lane. (D–E) Bar graphs exhibited the relative protein level of LC3A/β-actin, LC3B/β-actin (D) and BRF1/β-actin (E) by Western blot analysis.