Ouabain alleviates pathological changes. Representative lung histology of (A) HE staining, original magnifications 100 ×, Scale bar = 20 μm. (B) Masson’s trichrome original magnifications 100 ×, Scale bar = 20 μm. (C) Fibrosis scoring of the Masson trichrome staining on day 14, BLM group (black bar), BLM+Ouabain group (white bar) ,results are expressed as mean ± SEM, n = 4-6 mice per group, *P < 0.05. (D) The hydroxyproline content in lung tissue was significantly higher in the mice treated with BLM (no ouabian) than those treated with BLM+ouabain. results are expressed as mean ± SEM, n = 4-6 mice per group, *P < 0.05. (E) Assessment of the severity of pulmonary fibrosis on Ashcroft scale on day 14, BLM group (black bar), BLM+ouabian group (white bar), results are expressed as mean ± SEM, n = 4-6 mice per group, *P < 0.0.