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. 2018 Sep 15;10(9):2834–2847.

Figure 2.

Figure 2

Overexpression of miR-129-5p inhibits glioma cell proliferation in vitro. A. The relative expression level of miR-129-5p in U87-MG and LN229 cells was analyzed by qRT-PCR after transfection with lentivirus carrying has-miR-129-5p. (**P<0.01). B. Overexpression of miR-129-5p inhibited cell proliferation detected by CCK8 assays. Data are presented as the means of triplicate experiments. (**P<0.01). C, D. EdU assays show that miR-129-5p up-regulation inhibited cell proliferation in both U87 and LN229 cells. Representative images were shown (original magnification, 200×). (**P<0.01). E, F. The cell cycle phase of U87-MG and LN229 cells transfected with miR-129-5p or negative control (miR-ctrl) lentivirus analyzed by flow cytometry. (**P<0.01).