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. 2018 Sep 15;10(9):2834–2847.

Figure 5.

Figure 5

Down-regulation of DNMT3A inhibits glioma cell proliferation in vitro. A. U87-MG and LN229 cells were transfected with DNMT3A siRNA and cultured for in 96 h. Cell proliferation was determined using the CCK-8 assay. Data are presented as the means of triplicate experiments. (**P<0.01). B, C. Proliferating cells were examined using the EDU assay. Representative images are shown (original magnification, 200×). (**P<0.01). D, E. The cell cycle phase of U87-MG and LN229 cells transfected with DNMT3A siRNA or negative control (NC) was analyzed by flow cytometry. (**P<0.01). F. A heat map of relative expression of several DNMT3A-associated cell cycle genes in Rembrandt glioblastoma tissues sorted relative to the level of DNMT3A expression (r>0.4). G. Western blot analysis of DNMT3A, CDK2 and cyclinA2 in U87-MG and LN229 cells after knockdown of DNMT3A.