Figure 6.

Reintroduction of DNMT3A reverses the inhibitory effect of miR-129-5p. A. A rescue experiment was performed by introducing pcDNA3.1-DNMT3A or pcDNA3.1 in the presence or absence of ectopic miR-129-5p or miR-ctrl expression in U87-MG and LN229 cells. Western blot assays were performed to detect the levels of DNMT3A, CDK2 and cyclinA2 in the indicated cells. Actin was used as the loading control. B. Cell viability of glioma cells transfected with pcDNA3.1-DNMT3A and miR-129-5p separately or together was detected using the CCK-8 assay. C, D. Cell proliferative potential was evaluated using the EDU assay 48 h after co-transfection of pcDNA3.1-DNMT3A and miR-129-5p. E, F. Cell cycle distribution of glioma cells was measured using flow cytometry.