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LIPUS inhibits RANKL-induce expression of osteoclast-specific genes. BMMs were cultured with M-CSF (30 ng/mL) and RANKL (50 ng/mL) with or without LIPUS treatment for 0, 1, 3, or 5 days. The mRNA expression of osteoclast-specific genes (CTSK, TRAP, DC-STAMP, CTR, c-Fos, NFATc1) was measured using quantitative real-time PCR. Results were normalized to the expression of GAPDH. (*P < 0.05, **P < 0.01).
