Table 1.

P. marginatum Jacq and Ilex guayusa Loes total ethanol extract phytochemical analyses.

Secondary metabolite	Test	Piper marginatum Jacq	Ilex guayusa Loes
Alkaloids	Dragendorff	+	+
	Valser	+	+
	Mayer	+	+
	Wagner	+	+
Cardiotonics	Baljet	+	+
	Molisch	+	+
Carotenoids	Salkowiski	+	+
Coumarins	Ehrlich	−	+
	Fluorescence	−	+
Steroids	Liebermann–burchard	−	−
	Vanille- orthophosphoric acid	−	−
Flavonoids	Shinoda	+	+
	Rosenheim	−	−
	Leucoanthocyanidin	−	−
Sesquiterpenic lactones	Ferric hydroxamate	−	−
Saponins	Froth	−	−
	Rosenthaler	−	−
Tannins	Ferric chloride	+	+
Phenols	Gallic acid	+	+
Flavonoids	Quercetin	+	+

+: positive test result; −: negative test result.