Figure 2.

Construction and identification of STAT3-shRNA stably-transfected cells. (A) Fluorescence images of shRNA-transfected T24 cells (magnification, ×200). (B) Reverse transcription quantitative polymerase chain reaction was used to detect the level of STAT3 mRNA in STAT3-shRNA-T24 cell lines. (C) Western blot analysis was used to detect the expression of STAT3 protein in STAT3-shRNA-T24 cell lines. All data are presented as the mean ± standard deviation of the mean. **P<0.01. shRNA, short hairpin RNA; STAT3, signal transducer and activator of transcription.