Table I.
Effect of PPD12 on ADM-MDR reversal in KB/VCR and KB cells.
| IC50 ± SD (µM)a (fold reversalb) | |||
|---|---|---|---|
| Compound | Incubation time | KB | KB/VCR |
| ADM | 24 h | 2.3830±0.0304 (1.00) | 150.900±0.2891 (1.00) |
| 48 h | 0.3898±0.0373 (1.00) | 4.782±0.0476 (1.00) | |
| 72 h | 0.0713±0.0510 (1.00) | 1.521±0.0492 (1.00) | |
| 96 h | 0.0351±0.0736 (1.00) | 1.193±0.0540 (1.00) | |
| ADM+2.5 µM PPD12 | 24 h | 2.0640±0.0242 (1.15)c | 8.4310±0.0265 (17.90)f |
| 48 h | 0.5732±0.0265 (0.68) | 0.7795±0.0213 (6.13)e | |
| 72 h | 0.1493±0.0321 (0.48) | 0.2292±0.0328 (6.64)d | |
| 96 h | 0.0968±0.0241 (0.36) | 0.0692±0.0550 (17.24)d | |
a
IC50 ± SD (µM) values are represented as the mean ± SD of three independent experiments performed in triplicate.
b
The fold reversal of MDR was calculated by dividing the IC50 of cells with ADM and PPD12 by that with ADM alone.
c
P<0.05
d
P<0.01
e
P<0.001
f
P<0.0001 vs. the ADM group at the same time point. ADM, adriamycin; PPD12, 20(S)-protopanaxadiol derivative; SD, standard deviation.