Figure 5.

Knockdown of Z38 in AGS and MKN74 cells increased the protein expression levels of caspase-3 and caspase-9. AGS and MNK74 cells were treated with siZ38 for 72 h, and western blot analysis was performed to reveal the protein expression levels of caspase-3 and caspase-9. GAPDH was included as an internal control. Two bands of cl-caspase-3 were detected, but the top bands were specific to cl-caspase-3, the molecular weight of which is 17KD, and the lower one was also from cl-caspase-3, which has a smaller molecular weight (12KD). siZ38, Z38 small interfering RNA; cl-caspase, cleaved-caspase.