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. 2018 Oct 2;9:1123. doi: 10.3389/fphar.2018.01123

FIGURE 4.

FIGURE 4

Trans-Chalcone inhibits MSU-induced gp91phox mRNA expression and increases Nrf2 and Ho-1 mRNA expression. Mice were treated Trans-Chalcone (TC, 30 mg/kg, p.o., 100 μl) or vehicle (Tween 80 20% plus saline) 30 min before MSU (100 μg/10 μl/knee) or sterile saline (10 μl/knee) stimulus in the femur-tibial joint of swiss mice. Fifteen hours after MSU, knee joint samples were collected for the determination of the mRNA expression of: (A) gp91phox, (B) Nrf2, and (C) HO-1 by RT-qPCR. 3 × 106 BMDMs were seeded in 6 well plate and pre-treated with 3 μM of trans-chalcone 30 min before 500 ng/mL of LPS for priming over 3 h, followed by MSU (450 μg/mL) activation. After 5 h, the cells were collected for determination of mRNA expression of: (D) Nrf2 and (E) HO-1 by RT-qPCR. Results are expressed as mean ± SEM, data represent a total of 12 mice per group that were obtained in two independent experiment with 6 mice per experiment or the mean of 6 wells per group per experiment with three independent experiments for in vitro assays. (∗p < 0.05 vs. control group; #p < 0.05 vs. vehicle, one-way ANOVA followed by Tukey’s post-test).