Fig. 1.

cTAGE5 cKO in the brain leads to severe defects in development, which can be rescued by transgenic expression of human FLAG-cTAGE5. (A) Western blotting results showing the knockout of cTAGE5 in the cerebral cortex and cerebellum of Nestin-cKO mice at P20. Three independent replicates were performed. α-Tub was used as loading control. (B) Primary neurons at 14 div dissected from around E15.5 WT and cKO brains were stained with cTAGE5 antibody. (Scale bars, 5 μm.) Nucleus was stained with DAPI. (C) Body weight of WT and cKO mice from P7 to P20. t test; ***P < 0.001. (D) Postnatal survival curve of WT, cKO, and cKO-Res mice. One-way ANOVA; ***P < 0.001. (E) Measurement of body weight of WT, cKO, and cKO-Res mice at P20. One-way ANOVA; **P < 0.01; ***P < 0.001. (F and G) Images of the bodies (F) and brains (G) of the WT, cKO and cKO-Res mice at P20. (H and I) cKO mice showing abnormal limb clasping reflexes in tail suspension test. t test; ***P < 0.001. (J) The time of WT and cKO mice at P16 spent on the rotating rod (10 rotations per min) during Rota-rod test. t test; ***P < 0.001. Numbers of mice tested are labeled in C–E, I, and J.