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. 2018 Sep 17;115(40):E9449–E9458. doi: 10.1073/pnas.1804083115

Fig. 2.

Fig. 2.

cTAGE5 is expressed in neuron at the ER exit sites. (A) RT-PCR results showing different splicing isoforms in different tissues during the embryonic stages. The forward primer and reverse primer of “Primer 1” flank the spliced intron of cTAGE5, while “Primer 2” does not. 1: E14 brain; 2: E18 brain; 3: E14 primary neuron; 4: E18 kidney; 5: E18 liver; 6: E14 meninge. (B) Western blotting results showing the expression levels of cTAGE5 and p-CamKII (phospho T286) in the cerebral cortex at different developmental stages. GAPDH was used as loading control. (C and D) Primary neurons (14 div) dissected from E15.5 WT mouse cerebral cortex were stained with cTAGE5 (gray), MAP2 (green), and GFAP (red) antibodies (C); or cTAGE5, SAR1-GTP and MAP2 antibodies (D). (Scale bars, 20 μm in C and 2 μm in D.) Nucleus was stained with DAPI.