Table 1.

Comparison of membrane properties of evoked APs in fluorescent and nonfluorescent DCN neurons

PRV-152 fluorescent labeling	Vm, mV	Input resistance, MΩ	AP threshold, mV	Current required for evoked AP, nA	Latency, ms	Amplitude, mV	APD50, ms	APD50 rising, ms	APD50 falling, ms	AHP, mV	S1S2 interval, ms
Unlabeled	−46.45 ± 0.46	103.75 ± 8.18	−46.85 ± 0.88	0.08 ± 0.01	31.72 ± 5.32	68.01 ± 1.71	0.80 ± 0.04	0.32 ± 0.01	0.48 ± 0.03	−10.50 ± 0.67	17.37 ± 2.16
Labeled	−45.64 ± 0.97	79.63 ± 6.87	−48.30 ± 1.13	0.10 ± 0.02	40.04 ± 8.52	68.25 ± 1.86	0.72 ± 0.07	0.29 ± 0.02	0.43 ± 0.04	−11.96 ± 1.19	29.99 ± 9.13

Note that there were no statistical differences in any measures for evoked APs between fluorescence-labeled (n = 20) and unlabeled (n = 42) DCN neurons. Vm, resting membrane potential.