Fig. 6.

Effects of chloride on LPS-induced inflammasome activation in human PBMCs. (A) Immunoblot of DSS-crosslinked ASC oligomers and IL-1β from human PBMCs incubated in RPMI, PBS, or Cl⁻ free PBS ± LPS (1 µg⋅mL⁻¹) for 20 h (n = 5). (B) Immunoblot of ASC oligomers and caspase-1 from PBMCs incubated in PBS or Cl⁻ free PBS ± LPS (1 µg⋅mL⁻¹) and the NLRP3 inhibitor MCC950 (5 µM) for 20 h (n = 3). (C) Immunoblot of ASC oligomers and caspase-1 from PBMCs incubated in PBS or Cl⁻ free PBS ± LPS (1 µg⋅mL⁻¹) and the chloride channel inhibitor NPPB (100 µM) for 24 h (n = 2). (D) PBMCs were treated as in A ± nigericin (10 µM) added for the last 1 h of treatment (n = 2).