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Reconstitution of both scPaf1 and mtPaf1 complexes. (A) Schematic drawing of the subunits of the Paf1 complex. Known domains in Cdc73 and Rtf1 are labeled. Crystallized regions in this study for both scPaf1 and mtPaf1 subunits are color-coded. (B) Gel-filtration chromatogram of the scPaf1 complex. (C) SDS/PAGE analysis of the full-length five-subunit scPaf1 complex. (D) SDS/PAGE analysis of the Ctr9-truncated four-subunit mtPaf1 complex.
