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. 2018 Sep 13;115(40):E9391–E9400. doi: 10.1073/pnas.1808903115

Fig. 4.

Fig. 4.

MDM2 and Mycn promote cell-cycle entry of immature but not maturing murine cone precursors. (A) Scheme for lentiviral transduction of WT or RGP-MDM2 retinae with shRb1 or shSCR and with BE-Neo-MycnT58A or BE-Neo vector. (B) The number of YFP+,Arr3+ cells from P9 RGP-MDM2 explanted and shRb1- and Mycn-transduced retinae analyzed for Ki67 at 7 and 14 DIC. (C and D) Quantitation of YFP+,Rxrγ+ cells costained for Ki67 or EdU in P4 WT or RGP-MDM2 retinae at 7 (C) or 14 (D) DIC. Labels indicate oncogenic variables (shRb1, MDM2, Mycn) and omit the applied control vectors or WT genotype. (E) Quantitation of YFP+,Rxrγ+ cells or YFP+,Arr3+ cells costained for Ki67 or EdU in P4 RGP-MDM2 retinae transduced with shRb1 and MycnT58A and analyzed at 7, 14, and 21 DIC. Error bars indicate SD. Significance was assessed by ANOVA with Tukey’s HSD post hoc test (*P < 0.05; **P < 0.001, ns, not significant).