. 2018 Sep 17;115(40):10010–10015. doi: 10.1073/pnas.1805343115

Table 1.

HAT activity and AcCoA isothermal calorimetry binding data for Gcn5(67-317) and Ada2(1-120)/Gcn5(67-317) variants

Samples			Catalysis in 10 μM AcCoA				Catalysis at higher AcCoA concentrations				AcCoA binding
Ada2	Gcn5	sAB	[AcCoA], µM	k_cat, s⁻¹	K_m, µM	k_cat/K_M, M⁻¹⋅s⁻¹	[AcCoA], µM	k_cat, s⁻¹	K_m, µM	k_cat/K_M, M⁻¹⋅s⁻¹	AcCoA K_d, µM
−	+	−	10	0.048 ± 0.003	749 ± 112	65	100	0.102 ± 0.014	1,348 ± 400	75	7.8 ± 3
−	K223A	−	—	—	—	—	600	0.047 ± 0.003	802 ± 123	59	ND
+	+	−	10	1.28 ± 0.12	189 ± 62	6,790	24	1.27 ± 0.076	187 ± 38	6,780	2.3 ± 0.2
+	K223A	−	10	0.414 ± 0.029	1110 ± 175	372	600	0.430 ± 0.048	291 ± 103	1,480	NA
+	R89A	−	10	0.917 ± 0.085	364 ± 97	2,520	400	0.724 ± 0.078	187 ± 78	3,870	36.2 ± 5.5
+	R89A,K223A	−	10	0.096 ± 0.006	1930 ± 212	50	600	0.209 ± 0.023	771 ± 182	271	NA
D87A	+	−	10	1.06 ± 0.11	191 ± 76	5,570	30	1.15 ± 0.14	213 ± 97	5,420	2.7 ± 0.6
+	+	+	10	1.34 ± 0.096	156 ± 41	8,570	30	1.51 ± 0.16	156 ± 59	9,670	ND

Columns 4–7 show catalysis results in 10 μM AcCoA, columns 8–11 show catalysis results in near-saturating conditions of AcCoA, and column 12 shows AcCoA binding data. conc, concentration; NA, not applicable (because no binding detected); ND, not determined.