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The RppA biosensor is also functional in P. putida and C. glutamicum. Normalized signals generated from the RppA biosensors in (A) P. putida and in (B) C. glutamicum showed dose-dependent responses to intracellular malonyl-CoA abundance. Intracellular malonyl-CoA abundance was titrated using different concentrations of cerulenin added to the medium. Signals normalized with cell growth are plotted either with (RppA+) or without (RppA−) RppA expression. Error bars, mean ± SD (n = 3).
