Table 1.

Thermodynamics measurements (ITC) for binary and ternary complex formation between HBZ AD, KIX, and c-Myb

Ligand	Binding partner	Kd, µM	ΔG, kcal·mol−1	ΔH, kcal·mol−1	−TΔS, kcal·mol−1 T = 301K	ΔΔHternary-binary, kcal·mol−1	-TΔΔSternary-binary, kcal·mol−1
HBZ (3–77)	KIX*	1.0 ± 0.1	−8.2 ± 0.1	−14.9 ± 0.4	−6.3 ± 0.6	−4.2	−2.7
HBZ (3–77)	KIX:c-Myb*	0.042 ± 0.005	−10.2 ± 0.1	−19.1 ± 0.3	−9.0 ± 0.3	−4.2	−2.7
HBZ (3–56)	KIX*	2.4 ± 0.2	−7.7 ± 0.05	−11.1 ± 0.1	−3.5 ± 0.2	−4.6	−2.4
HBZ (3–56)	KIX:c-Myb*	0.11 ± 0.01	−9.6 ± 0.1	−15.7 ± 0.2	−5.9 ± 0.2	−4.6	−2.4
HBZ (3–36)	KIX*	8.2 ± 0.6	−7.0 ± 0.04	−2.4 ± 0.2	4.6 ± 0.3	−4.2	−2.2
HBZ (3–36)	KIX:c-Myb*	0.34 ± 0.05	−8.9 ± 0.1	−6.5 ± 0.9	2.4 ± 0.9	−4.2	−2.2
c-Myb	KIX†	0.19 ± 0.003	−9.3 ± 0.01	−3.2 ± 0.02	−6.1 ± 0.02	−6.6	−5.4
c-Myb	KIX:AD1†	0.029 ± 0.004	−10.4 ± 0.01	−9.8 ± 0.1	0.63 ± 0.08	−6.6	−5.4

^*The K_d, ΔH, and −TΔS values are the average and SD of triplicate experiments. ΔG values were calculated with ΔG = −RTlnK_d. All measurements were taken at T = 301 K.

^†Thermodynamic values are the average and SD of duplicate experiments.