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. 2018 Sep 19;115(40):E9381–E9390. doi: 10.1073/pnas.1810133115

Fig. 5.

Fig. 5.

Expression of NLRP3 inflammasome components and the mature form of IL-1β. (A) LAD2 cells (1 × 106 cells per well) were seeded in a 12-well culture plate and were stimulated with SP (1 μΜ), IL-33 (30 ng/mL), or their combination for 24 h. Cell lysates were collected after 24 h, and protein levels of the NLRP3 inflammasome components (NLRP3, ASC, caspase-1), pro-IL-1β, and active IL-1β (p17) were measured by Western blot, using β-actin as loading control (shown in a representative gel of n = 3). SP and IL-33 increase caspase-1 gene expression. (B) LAD2 cells (1 × 106 cells per well), (n = 3, **P < 0.01 compared with unstimulated cells). (C) hCMBCs (0.3 × 106 cell per well) were seeded in a 12-well culture plate and were stimulated by SP (1 µM), IL-33 (30 ng/mL), or their combination for 6 h. Caspase-1 gene expression was measured by qRT-PCR and normalized to human GAPDH endogenous control (n = 3, ***P < 0.001 compared with unstimulated cells).