Fig. 2.

Vortex-based cell release and step emulsification. (A) The flow rate decays quickly when the pressure is released to dissipate the vortices. Beads tracked in the exit region of the vortex were used to calculate the decay. (B) Droplet generation remains stable and monodisperse at flow rates from 0.001 to 0.002 mL/min per channel but becomes polydisperse beyond these flow rates. (C) There is minimal loss between the cells captured in the vortex device and the cells in the droplets, because of the continuous flow integrated system.