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. Author manuscript; available in PMC: 2019 Dec 11.
Published in final edited form as: Anal Chim Acta. 2018 Mar 10;1037:274–280. doi: 10.1016/j.aca.2018.02.057

Figure 1. Sulfur Metabolism and Tagging Regime.

Figure 1

A. Structure and molecular weight of thiols that participate in the sulfur metabolic pathway. B. Alkyl maleimides are reacted with reduced thiol in 80% acetonitrile solution with 5 mM ammonium formate at pH 8. Tagged thiols were combined at 1:1 ratio and analyzed by HILIC-MS/MS. The mass tags yield differing molecular weights but identical retention times by HILIC separation.