In vivo activity of chemically synthesized arabinosylated CLV3 peptides.
(A) HPLC purification of the chemically synthesized [Ara3]CLV3 peptide.
(B) Selected ion at m/z 1877.780 that corresponds to the 13-amino-acid [Ara3]CLV3 peptide in MS analysis.
(C) Similar root growth inhibition mediated by MCLV3p and [Ara3]CLV3p. Ler seedlings were treated with synthetic peptides (0, 1, 10, 100, 1000, 10,000 nM) for 14 days. Error bars represent SD from repeats (n = 10). ** indicates significant difference (two-way ANOVA test, p<0.001). n.s., not significant.
(D, E) CLV2-dependent root growth inhibition mediated by [Ara3]CLV3p and MCLV3p. WT Ler and clv2-1 mutant seedlings were treated without or with 30 nM, 100 nM and 1000 nM [Ara3]CLV3p or MCLV3p for 7 days. Scale bar = 10 mm.