Figure 5.

Comparing Murine and Human Colonic Mesenchymal Cells

(A) Confusion matrices of human (left) and mouse (right) random forest models applied to independent datasets from the same species and different species show the proportion of real and model-predicted cell cluster identities for healthy control (HC), human UC, or mouse DSS.

(B) Human HC model features scored for cluster specificity in human (hS1, hS2, hS3) and mouse (mS1, mS2, mS3) data. The heatmap shows increasingly positive cluster markers in yellow (>0.5) and increasingly negative cluster markers in purple (<0.5), and non-specific genes in green ( = 0.5). The bar plot shows the correlation between mouse and human marker specificity for each cluster.

(C) Examples of features that drive the random forest results: MFAP4, IGFBP3, and SOX6.

(D) Human and mouse cluster marker gene overlap correlation heatmap.

(E) t-SNE plot visualizing sub-cluster analysis of S2 cells from healthy mouse scRNA-seq. Two distinct cell clusters, not previously detected, show similarities to human S2a and S2b counterparts.

(F) Wnt5a expression by both S2a- and S2b-like mouse sub-clusters.

(G) Violin plots show example S2 markers identified from human data that do not exhibit a conserved expression patterns in mouse S2 subtypes.