FIGURE 3.

FOS-supplementation worsened barrier function. (A) Time to activation of a HEK-Blue cell system sensitive to LPS by serum. A lower time to activation of this cell system is indicative of higher levels of serum LPS. Presented as mean time to activation ± SEM. FOS-supplementation was significantly associated with increased LPS (P = 0.05) (Two-way ANOVA). (B) Relative mRNA expression of occludin following logarithmic transformation in the TI of mice presented as mean expression ± SEM. Both FOS-supplementation (P = 0.05) and ICR (P < 0.01) were associated with decreased expression. (C) Relative mRNA expression of ZO1 in the TI of mice presented as mean expression ± SEM. Both FOS-supplementation (P = 0.04) and ICR (P = 0.04) were associated with decreased expression.

Control: n = 5; Control-FOS: n = 6; ICR-C: n = 5; ICR-FOS: n = 6