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. 2018 Sep 15;7(9):bio031856. doi: 10.1242/bio.031856

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Survival of acid-challenged strains. The percentage survival of strains challenged at pH 4.9 for 1 h was calculated compared with the count at time zero. Plasmids are denoted below the x-axis: none, no plasmid; pBlue, empty vector; pBlue+G, empty vector with glutamate in the medium, pGadA, pGadBC, pGadE, pHdeAB and pUdhA. IPTG and glutamate were supplemented in the culture media. The survival of ZG3 (icdA^NADPΔudhA) was significantly increased (P<0.05) by the expression of pHdeAB, pGadA, pGadBC, pGadE or pUdhA compared with ZG3 with or without pBlue. The survival of ZG1 (icdA^NADP, WT) and ZG4 (icdA^NADΔudhA) was not significantly affected by the plasmids (P>0.05). Data are presented as the mean±s.d. of three independent experiments.