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. 2018 Sep 15;7(9):bio031856. doi: 10.1242/bio.031856

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — The effect of NADH on the WT and ZG3 strains. (A) Growth curves for ZG1 (icdA^NADP, WT) and ZG3 (icdA^NADPΔudhA) with or without NADH supplementation. ZG1-NADH and ZG3-NADH indicate that NADH was added to the culture medium at the beginning. The dashed line represents the long lag phase of ZG3. The growth rate of ZG3-NADH was significantly faster than that of ZG3 (P<0.05). (B) Q-PCR results regarding gene expression levels in ZG3-NADH compared with ZG1-NADH. (C) Acid resistance assay for ZG1 and ZG3 cells with or without NADH supplementation. Data are presented as the mean±s.d. of three independent experiments. *P<0.05. **P<0.01.